Composite

Part:BBa_K4949010:Design

Designed by: UManitoba_2023   Group: iGEM23_UManitoba   (2023-10-11)


This composite part consists of all components required for expressing RPA1511


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 333
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 159
    Illegal NgoMIV site found at 168
    Illegal NgoMIV site found at 394
    Illegal NgoMIV site found at 568
    Illegal NgoMIV site found at 628
    Illegal AgeI site found at 193
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 871


Design Notes

The sequence is codon optimized for Expression in E.coli


Source

RPA1511 is a carboxyl esterase originally identified in Rhodopseudomonas palustris.

References