![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4949010:Design
Designed by: UManitoba_2023 Group: iGEM23_UManitoba (2023-10-11)
This composite part consists of all components required for expressing RPA1511
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 333
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 159
Illegal NgoMIV site found at 168
Illegal NgoMIV site found at 394
Illegal NgoMIV site found at 568
Illegal NgoMIV site found at 628
Illegal AgeI site found at 193 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 871
Design Notes
The sequence is codon optimized for Expression in E.coli
Source
RPA1511 is a carboxyl esterase originally identified in Rhodopseudomonas palustris.